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Rapid screening of lactic acid bacteria for restriction endonuclease activity

✍ Scribed by Mark T. Poch; George A. Somkuti


Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
426 KB
Volume
7
Category
Article
ISSN
0951-208X

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A method is described which allows a large number of bacterial strains to be rapidly and easily screened for the presence of site-specific endonucleases. The method involves selective permeabilization of the bacterial cell and analysis of the exuded material. Type II restriction endonucleases from c