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Purification of the restriction endonuclease PalI

✍ Scribed by Krishna Baski; George W. Rushizky

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
514 KB
Volume
99
Category
Article
ISSN
0003-2697

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✦ Synopsis

The restriction endonuclease Pal1 was purified from Providencia alcalifaciens 1650-fold with a yield of 33%. The purified protein moved as a single band upon polyacrylamide gel electrophoresis. When this was carried out in the presence of sodium dodecyl sulfate, a molecular weight of 31,000 was obtained for PalI. Gel filtration through Sephacryl S200 gave molecular weights ranging from 44,000 to 53,000 when 58 to 1870 nglml enzyme were used, respectively. Other properties of the enzyme are described.

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