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Photochemical Regulation of Restriction Endonuclease Activity

โœ Scribed by Douglas D. Young; Jeane M. Govan; Mark O. Lively; Alexander Deiters

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
416 KB
Volume
10
Category
Article
ISSN
1439-4227

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โœฆ Synopsis

Abstract

Removal by the light: The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation. Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.magnified image

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