Restriction endonuclease mapping of pSC101 and pMB9
β Scribed by Tait, Robert C. ;Boyer, Herbert W.
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 341 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
A restriction endonuclease analysis of the plasmids pSC101 and pMB9 has allowed a determination of the alterations that occurred in the tetracycline resistance locus during the construction of pMB9 from pSC101. The genes for four of the polypeptides involved in tetracycline resistance have been positioned on the restriction endonuclease map of pSC101.
π SIMILAR VOLUMES
A detailed restriction endonuclease cleavage map of the plasmid pKM101 has been constructed. pKM101 plasmids containing individual Tn5 insertions were used to facilitate the ordering of restriction fragments generated by enzymes cleaving pKM101 at multiple sites. By restriction enzyme analysis, pKM1
## Abstract **Removal by the light**: The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonucleas