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Rapid electroelution of nucleic acids from agarose and acrylamide gels

✍ Scribed by H.Peter Zassenhaus; Ronald A. Butow; Yolanda P. Hannon

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
880 KB
Volume
125
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe a procedure for the electroelution of nucleic acids from agarose or acrylamide gels. The method employs a combination of low-and high-ionic-strength buffers so that nucleic acids electroelute under a high-voltage gradient but accumulate over a high-salt barrier. By this means electroelution is rapid (1-2 h) and recovery is quantitative (at least 78%). DNA fragments as small as 47 bp and as large as 49 kbp can be efficiently electroeluted. Moreover, the entire apparatus can be treated with diethylpyrocarbonate to inactivate RNase so that RNA can be recovered intact. The apparatus is commercially available, but a homemade device of similar construction can be easily fabricated.

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