An ethidium-acrylamide affinity medium for recovery of nucleic acids from free solution and from polyacrylamide and agarose gels

✍ Scribed by Ann Treece Vacek; Don P. Bourque; N.Gail Hewlett

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 683 KB
• Volume: 124
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90058-6

✦ Synopsis

The simple preparation of an ethidium-bromide-based nucleic acid affinity medium is described. The medium is composed of an acrylamide matrix to which ethidium bromide is attached. Its use in preparative purification and fractionation of nucleic acids in solution and in electrophoretic elution of nucleic acids from gels is reported. Nucleic acids can be eluted from this medium with a buffered salt solution and concentrated by ethanol precipitation without persistent contamination with undesirable impurities.