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Electrophoretic extraction-concentration of ribonucleic acid from agarose-acrylamide composite gels

✍ Scribed by Mohammad A. Rashid; Shuichi Hashizume

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 486 KB
Volume: 127
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90183-x

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✦ Synopsis

A simple technique for the electrophoretic extraction of RNA, under constant monitoring by bromphenol blue line, in a concentrated form from agarose-a&amide composite gels run according to A. C. Peacock and C. W. Dingman, (Biochemistry 7,668-674, (1968)) has been developed. Typically, the extraction time from 5 gel slices/tube containing 56 pg of RNA was 3.5 h, and the maximum concentration of the recovered RNA was 0.37 mg/ml with a total recovery of more than 90%. The desired scale can be adjusted by manipulating the number of tubes or number of slices.

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Electrophoretic extraction-concentration

Electrophoretic extraction-concentration of proteins from polyacrylamide gels under alkaline, neutral and acidic conditions

✍ Shuichi Hashizume; Mohammad A. Rashid; Masahiro Shoji; Kazuhiko Kuroda 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 487 KB

## Electrophoretic extraction-concentration of proteins from polyacrylamide gels under alkaline, neutral and acidic conditions Morinaga Institute of Biological Science, Yokohama A simple technique has been developed for the electrophoretic extraction of milligram to microgram quantities of protein