Approximately one-third of all proteins reported in the literature have a pI sufficiently high to be resolved by cation-exchange chromatography. This paper reports the preparation and use of new high-performance polymeric-bonded-phase cation-exchange columns. Starting from a very stable, covalently
Rapid cation-exchange chromatography of hemoglobins and other proteins
โ Scribed by David J. Burke; J.Keith Duncan; Chris Siebert; Gary S. Ott
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 429 KB
- Volume
- 359
- Category
- Article
- ISSN
- 1873-3778
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The use of a recently developed cation-exchange HPLC packing material for the separation of hemoglobin types in human blood has been investigated. Adult and newborn hemolysates from normal individuals and from subjects with hemoglobin disorders were analyzed using a weak cation carboxymethyl-bonded