The use of a recently developed cation-exchange HPLC packing material for the separation of hemoglobin types in human blood has been investigated. Adult and newborn hemolysates from normal individuals and from subjects with hemoglobin disorders were analyzed using a weak cation carboxymethyl-bonded
Separation of Hemoglobin Acetaldehyde Adducts by High-Performance Liquid Chromatography Cation-Exchange Chromatography
โ Scribed by L. Itala; K. Seppa; U. Turpeinen; P. Sillanaukee
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 496 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0003-2697
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Approximately one-third of all proteins reported in the literature have a pI sufficiently high to be resolved by cation-exchange chromatography. This paper reports the preparation and use of new high-performance polymeric-bonded-phase cation-exchange columns. Starting from a very stable, covalently