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Separation of asymmetrical hybrid hemoglobins by anaerobic cation-exchange high-performance liquid chromatography

โœ Scribed by Charlotte Yu Ip; Toshio Asakura


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
388 KB
Volume
139
Category
Article
ISSN
0003-2697

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## Asymmetrical hybrid hemoglobins formed from mixtures of oxyhemoglobins S and F and A and F were separated by high-performance liquid chromatography on a 4.6 X 250 mm wide-pore polyethyleneimine-silica gel column under anaerobic conditions. The resulting HPLC chromatogram showed three peaks, wit

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The use of a recently developed cation-exchange HPLC packing material for the separation of hemoglobin types in human blood has been investigated. Adult and newborn hemolysates from normal individuals and from subjects with hemoglobin disorders were analyzed using a weak cation carboxymethyl-bonded