Approximately one-third of all proteins reported in the literature have a pI sufficiently high to be resolved by cation-exchange chromatography. This paper reports the preparation and use of new high-performance polymeric-bonded-phase cation-exchange columns. Starting from a very stable, covalently
โฆ LIBER โฆ
Variables in the high-pressure cation-exchange chromatography of proteins
โ Scribed by Charles A. Frolik; Linda L. Dart; Michael B. Sporn
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 670 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0003-2697
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The chromatographic behavior of a number of globular proteins was studied on a Bio-Sil TSK CM-2-SW weak cation exchange HPLC column under acidic conditions. A linear gradient of O-I M NH4Ac in I M HOAc, inducing a convex pH gradient from 2.4-4.8, resulted in an excellent separation of highly basic p