Approximately one-third of all proteins reported in the literature have a pI sufficiently high to be resolved by cation-exchange chromatography. This paper reports the preparation and use of new high-performance polymeric-bonded-phase cation-exchange columns. Starting from a very stable, covalently
โฆ LIBER โฆ
Recovery of proteins in high performance ion exchange chromatography
โ Scribed by Kato, Y. ;Nakamura, K. ;Hashimoto, T.
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 155 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0935-6304
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That the macroporous anion-exchange resin AG MP-1 can be used with HPLC equipment and common aqueous buffers for the chromatography of proteins is shown. The utility of this system is illustrated by the partial purification and complete resolution of the three protein synthesis elongation factors fr