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Raman spectroscopy in investigations of mechanism of human serum albumin denaturation under action of sodium dodecyl sulfate

✍ Scribed by I.M. Vlasova; D.V. Polyansky; A.M. Saletsky

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 78 KB
Volume: 4
Category: Article
ISSN: 1612-2011
DOI: 10.1002/lapl.200710070

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✦ Synopsis

The mechanism of denaturation of human serum albumin under action of an anionic detergent – sodium dodecyl sulfate (SDS) is investigated by Raman spectroscopy method. The quantitative contents of α-helical segments and random packing segments of polypeptide chain of albumin at different concentrations of SDS and at different pH values of solutions are determined. It is shown, that more intensive denaturation under action of SDS, consisting in disturbance of secondary and tertiary structure of albumin, takes place at pH values of solution, smaller the isoelectric point of protein.

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