𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Raman spectroscopy in comparative investigations of mechanisms of binding of three molecular probes – fluorescein, eosin, and erythrosin – to human serum albumin

✍ Scribed by I.M. Vlasova; A.M. Saletsky

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
116 KB
Volume
5
Category
Article
ISSN
1612-2011

No coin nor oath required. For personal study only.

✦ Synopsis

The comparative analysis of binding of three molecular fluorescent probes (fluorescein, eosin, and erythrosin), belonging to one homologous family, to human serum albumin (HSA) is made by Raman spectroscopy method. The binding of all three probes to binding Center I of HSA is registered. The character of binding of initial probe of the given homologous family -fluorescein -to protein differs from character of binding of its halogen-derivatives (eosin and erythrosin) to protein.

The differences in binding of these three probes to HSA are determined by value of electronegativity of atoms of lateral radicals in structural formulas of probes and, therefore, by value of pK of their ionized groups.

Raman intensity, 10-5 a.u.

📜 SIMILAR VOLUMES

Raman spectroscopy in investigations of
Raman spectroscopy in investigations of mechanism of binding of human serum albumin to molecular probe fluorescein
✍ I.M. Vlasova; A.M. Saletsky 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 107 KB

The mechanism of binding of molecular probe fluorescein to molecules of human serum albumin was studied by the Raman spectroscopy method. The position of binding Center on human serum albumin molecule for fluorescein is determined. The amino acid residues of albumin molecule, participating in bindin

Investigation of mechanism of binding of
Investigation of mechanism of binding of molecular probe eosin to human serum albumin by Raman spectroscopy method
✍ I. M. Vlasova; D. V. Polyansky; A. M. Saletsky 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 113 KB

The mechanism of binding of molecular probe eosin to molecules of human serum albumin was studied by the Raman spectroscopy method. The position of binding center on human serum albumin molecule for eosin is determined. The amino acid residues of albumin molecule, participating in binding of eosin a