Quantitative analysis of polyethylene glycol (PEG) in PEG-modified proteins/cytokines by aqueous two-phase systems
β Scribed by Cristina Delgado; Farooq Malik; Barbara Selisko; Derek Fischer; Gillian E. Francis
- Book ID
- 116098265
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 775 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0165-022X
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π SIMILAR VOLUMES
The electrochemical effect of a charged dextran derivative and the hydrophobic effect of hydrophobic chain PEG derivative on partitioning of six types of proteins in PEGjdextran aqueous two-phase systems were investigated. When 1. 6%(w/u)DEAE-dextran was present in the system ,the partition coeffici
Cd(II) plus iodide species were extracted into PEG-rich phases in the aqueous PEG(1550)-(NH 4 ) 2 SO 4 system at pH 2.05 -7.12. IR spectra show that increasing (NH 4 ) 2 SO 4 solution acidity does not protonate PEG ether oxygen atoms, but decreases water content in the PEG-rich phases. Metallic spec
PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity