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Partition of proteins in aqueous two-phase systems containing charged dextran or hydrophobic PEG

✍ Scribed by Xijun Chai; Meifang Jin; Chengzhang Jiang

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
362 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

The electrochemical effect of a charged dextran derivative and the hydrophobic effect of hydrophobic chain PEG derivative on partitioning of six types of proteins in PEGjdextran aqueous two-phase systems were investigated. When 1. 6%(w/u)DEAE-dextran was present in the system ,the partition coefficient decreased quickly with increasing pH value; when 0. 3% (w/w )PEG pentadecanoic acid ester was present in the system. the partition coefficient of protein with strong hydrophobiclty was greatly increased. The experimental results show that the influence of hydrocarbon chain PEG derivative on partition coefficient is closely related to the hydrophobicity of proteins-

πŸ“œ SIMILAR VOLUMES

Contribution of protein charge to partit
Contribution of protein charge to partitioning in aqueous two-phase systems
✍ Weiyu Fan; Ufuk Bakir; Charles E. Glatz πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 178 KB πŸ‘ 2 views

Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating