An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecu
Partitioning of β-galactosidase fusion proteins in PEG/potassium phosphate aqueous two-phase systems
✍ Scribed by Kristina Köhler; Andres Veide; Sven-Olof Enfors
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 618 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0141-0229
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The electrochemical effect of a charged dextran derivative and the hydrophobic effect of hydrophobic chain PEG derivative on partitioning of six types of proteins in PEGjdextran aqueous two-phase systems were investigated. When 1. 6%(w/u)DEAE-dextran was present in the system ,the partition coeffici
PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity