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Fusarium solani pisirecombinant cutinase partitioning in peg/potassium phosphate aqueous two-phase systems

✍ Scribed by M. J. Sebastião; J. M. S. Cabral; M. R. Aires-Barros

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
289 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecular weight, polymer concentration and pH were carried out using a purified preparation of the cutinase. The enzyme partition coefficient was enhanced with decreasing PEG molecular weight, increasing tie-line length and PH.

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