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PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems

✍ Scribed by B. A. Andrews; D. M. Head; P. Dunthorne; J. A. Asenjo

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
366 KB
Volume
4
Category
Article
ISSN
0951-208X

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✦ Synopsis

PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity of protein separation in aqueous two-phase systems.

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