A solution of ethyl chloroformate + trifluoroethanol (or ethanol) + pyridine reacts rapidly with carboxylic and amino groups of amino acids to form n-ethoxycarbonyl amino acid trifluoroethyl (or ethyl) esters, which produce strong peaks representative of molecular ions in both positive and negative
Quantitative analysis of fluorinated ethylchloroformate derivatives of protein amino acids and hydrolysis products of small peptides using chemical ionization gas chromatography-mass spectrometry
β Scribed by Ping Cao; Mehdi Moini
- Book ID
- 108339880
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 500 KB
- Volume
- 759
- Category
- Article
- ISSN
- 1873-3778
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## Abstract Highβperformance liquid chromatography (HPLC) coupled to atmospheric pressure chemical ionization (APCI) mass spectrometry was used for the separation and detection of amino acid and peptide enantiomers. With detection limits as low as 250 pg, 25 amino acids enantiomers were baseline re
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