Characterization of fluorinated ethylchloroformate derivatives of protein amino acids using positive and negative chemical ionization gas chromatography/mass spectrometry

✍ Scribed by Mahnaz Vatankhah; Mehdi Moini

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 395 KB
• Volume: 23
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200230507

✦ Synopsis

A solution of ethyl chloroformate + trifluoroethanol (or ethanol) + pyridine reacts rapidly with carboxylic and amino groups of amino acids to form n-ethoxycarbonyl amino acid trifluoroethyl (or ethyl) esters, which produce strong peaks representative of molecular ions in both positive and negative chemical ionization gas chromatographylmass spectrometry. Sensitivity of detection of the trifluoroethyl ester derivatives versus ethyl ester derivatives in the positive and negative chemical ionization modes is also discussed.