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Quantitation of Target Molecules from Polymerase Chain Reaction-Based Limiting Dilution Assays

✍ Scribed by RODRIGO, ALLEN G.; GORACKE, PAUL C.; ROWHANIAN, KIARASH; MULLINS, JAMES I.

Book ID
121829334
Publisher
Mary Ann Liebert
Year
1997
Tongue
English
Weight
1014 KB
Volume
13
Category
Article
ISSN
0889-2229

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Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference