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Quantitative determination of tumor cell intravasation in a real-time polymerase chain reaction-based assay

✍ Scribed by Emilia Mira; Rosa Ana Lacalle; Concepción Gómez-Moutón; Esther Leonardo; Santos Mañes


Book ID
110338097
Publisher
Springer
Year
2002
Tongue
English
Weight
150 KB
Volume
19
Category
Article
ISSN
0262-0898

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Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference