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Real-Time Polymerase Chain Reaction Based Assays for Quantitative Detection of Barley, Rice, Sunflower, and Wheat

✍ Scribed by Hernández, Marta; Esteve, Teresa; Pla, Maria

Book ID
111929495
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
143 KB
Volume
53
Category
Article
ISSN
0021-8561

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Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference