A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics: Volume 302, Number 1 (2002), pages 52–59

A New Quantitative Method of Real Time R

A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics

✍ Weihong Liu; David A. Saint 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference