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Quantitative detection of Borrelia burgdorferi with a microtiter-based competitive polymerase chain reaction assay*

✍ Scribed by GERMER, J; RYCKMANN, B; MORO, M; HOFMEISTER, E; BARTHOLD, S; BOCKENSTEDT, L; PERSING, D


Book ID
122613121
Publisher
Adis International Limited (now part of Wolters Kluwer Health)
Year
1999
Tongue
English
Weight
860 KB
Volume
4
Category
Article
ISSN
1532-8619

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Aequorin-based flash-type bioluminescent methods can detect nucleic acid molecules in the attomolar range (10(-18)) enabling improved monitoring of the polymerase chain reaction (PCR) at cycles previously considered too low for product detection. The high sensitivity of bioluminescence (BL) was used