A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.
Quantitation of nanogram amounts of protein using [3H]dinitrofluorobenzene
โ Scribed by R.M. Schultz; J.D. Bleil; P.M. Wassarman
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 179 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0003-2697
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