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Quantitation of microgram amounts of protein in SDS-mercaptoethanol-tris electrophoresis sample buffer

✍ Scribed by Robert W. Rubin; Robert W. Warren


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
293 KB
Volume
83
Category
Article
ISSN
0003-2697

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Quantitation of proteins solubilized in
✍ Zahur Zaman; Robrecht L. Verwilghen πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 405 KB

A simple method for determination of proteins, initially solubilized in Tris buffer containing sodium dodecyl sulfate (SDS), mercaptoethanol, and sucrose, is described. This method is based on protein and Coomassie brilliant blue G-250 binding but it involves the removal of excess SDS by precipitati