✦ LIBER ✦

Fluorometric determination of selenium in nanogram amounts in biological materials using 2,3-diaminonaphthalene

✍ Scribed by Ryoichi Hasunuma; Tadao Ogawa; Yasuhiro Kawanishi

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 213 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90510-3

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✦ Synopsis

Nanogram amounts of selenium in biological material were determined fluorometrically using 2,3-diaminonaphthalene after digestion with nitric-perchloric acids. Linear relationship between fluorescence and selenium concentration held within 100 ng of selenium, and the experimental scattering was less than 4% for a range from 5 to 25 ng of selenium. A recovery of 99 k 5% was achieved for 2.5 ng of selenium preadded to tuna muscle extract. The method is characterized by simple procedures and is suited for small quantities of samples.

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