Quantitation of hemoglobin biosynthesis with agarose isoelectric focusing
โ Scribed by Anne G. Leary; Pamela N. Porter; Makio Ogawa
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 473 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0361-8609
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๐ SIMILAR VOLUMES
## Quantitation of glycated hemoglobins in human adult blood by capillary isoelectric focusing A precise and reproducible method for assessment of glycated hemoglobin in human adult red blood cells is reported, based on capillary isoelectric focusing (IEF). In order to obtain baseline resolution b
Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer.