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Direct spectrophotometric analysis of hemoglobin in isoelectric focusing tube gels

✍ Scribed by Richard S. Magliozzo; Constance M. Park; Jack Peisach

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 648 KB
Volume: 140
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90165-9

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✦ Synopsis

Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer. Direct optical sampling of gels allowed the identification of focused hemoglobin valency hybrids which contain two oxidized monomers per tetramer.

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