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Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction

โœ Scribed by Donate Zipeto; Fausto Baldanti; Davide Zella; Milena Furione; Ada Cavicchini; Gabriele Milanesi; Giuseppe Gerna


Book ID
119115247
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
835 KB
Volume
44
Category
Article
ISSN
0166-0934

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Human cytomegalovirus infection during c
โœ Motohiro Shibata; Tsuneo Morishima; Makoto Terashima; Hiroshi Kimura; Kiyotaka K ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 858 KB

Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV) DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the immediate early 1 and the late antigen genes of CMV, respectively. Either primer sets could detect as few as 0.01 plaque-forming