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A novel, internally competitive polymerase chain reaction for quantification of Human Cytomegalovirus DNA in human leukocytes

โœ Scribed by OL Caballero; CLP Menezes; LL Villa; AJG Simpson


Book ID
115638337
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
266 KB
Volume
13
Category
Article
ISSN
0890-8508

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The development of a quadriplex PCR method with amplification of HCMV in a single-step procedure using primers taken from four different regions of the viral genome is described. Different concentrations of dNTPs and MgCl 2 were assayed in order to optimize the constitution of the buffer for the mul