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Failure to detect human cytomegalovirus DNA in peripheral blood leukocytes of healthy blood donors by the polymerase chain reaction

โœ Scribed by A. Bitsch; H. Kirchner; R. Dupke; G. Bein


Book ID
114439411
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
818 KB
Volume
32
Category
Article
ISSN
0372-1248

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Human cytomegalovirus infection during c
โœ Motohiro Shibata; Tsuneo Morishima; Makoto Terashima; Hiroshi Kimura; Kiyotaka K ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 858 KB

Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV) DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the immediate early 1 and the late antigen genes of CMV, respectively. Either primer sets could detect as few as 0.01 plaque-forming