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Human Cytomegalovirus DNA Is Not Detectable with Nested Double Polymerase Chain Reaction in Healthy Blood Donors

โœ Scribed by Noriko Urushibara; Kil-Won Kwon; Tsuneo A. Takahashi; Sadayoshi Sekiguchi


Book ID
115110327
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
772 KB
Volume
68
Category
Article
ISSN
0042-9007

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Human cytomegalovirus infection during c
โœ Motohiro Shibata; Tsuneo Morishima; Makoto Terashima; Hiroshi Kimura; Kiyotaka K ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 858 KB

Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV) DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the immediate early 1 and the late antigen genes of CMV, respectively. Either primer sets could detect as few as 0.01 plaque-forming