Detection of human cytomegalovirus DNA in peripheral blood leukocytes by the polymerase chain reaction
โ Scribed by Ian.S. Bevan; Matthew R. Walker; Richard A. Daw; Andreas Bitsch; Gregor Bein; Regina Dupke; Holger Kirchner
- Book ID
- 114439665
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 244 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0372-1248
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Diagnosis of significant infections by human herpesvirus 6 (HHV6) and 7 (HHV7) in transplant patients has proved difficult because both viruses are ubiquitous and can cause persistent infections in their hosts. The significance of viral DNA detected in peripheral blood leukocytes (PBLs; DNAemia) by
Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV) DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the immediate early 1 and the late antigen genes of CMV, respectively. Either primer sets could detect as few as 0.01 plaque-forming