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Purification of T4 RNA ligase by 2′, 5′-ADP sepharose chromatography

✍ Scribed by M. Sugiura; M. Suzuki; E. Ohtsuka; S. Nishikawa; H. Uemura; M. Ikehara

Book ID
115909345
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
277 KB
Volume
97
Category
Article
ISSN
0014-5793

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✍ Inger Carlberg; Bengt Mannervik 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 454 KB

Glutathione reductase has been purified to at least 98% homogeneity from calf liver. An essential part in the procedure involves affinity chromatography on 2',5'-ADP-Sepharose 4B to which the enzyme remains bound in the presence of 0.4 M phosphate. This step separates glutathione reductase from the