𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of mannuronan C-5-epimerase by affinity chromatography on alginate-Sepharose

✍ Scribed by Gudmund Skjåk-Br˦k; Bjørn Larsen

Book ID
108309614
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
197 KB
Volume
103
Category
Article
ISSN
0008-6215

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Biosynthesis of alginate: Purification a
Biosynthesis of alginate: Purification and characterisation of mannuronan C-5-epimerase from azotobacter vinelandii
✍ Gudmund Skj»k-Bræk; Bjørn Larsen 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 594 KB

A mannuronan C-5-epimerase from the soil bacterium Azotobacter vinelundii has been highly purified and characterised. The enzyme is a single polypeptide chain with a mobility in gradient and SDS electrophoresis corresponding to a mol. wt. of 122,000; it has a p1 of 4, highest activity at pH 7.0, and