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Purification of glycogen phosphorylase by affinity chromatography on 5′-AMP sepharose

✍ Scribed by Niels B. Sørensen; Palle Wang


Book ID
118310327
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
248 KB
Volume
67
Category
Article
ISSN
0006-291X

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A sensitive method for measuring phosphorylase kinase activity by the incorporation of anP from ]y-"\*]ATP into phosphorylase in the presence of other phosphorylation reactions is described. The kinase reaction is carried out in a crude homogenate. After stopping the reaction, a portion of the react