Purification and characterization of glu
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Inger Carlberg; Bengt Mannervik
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Article
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1981
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Elsevier Science
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English
⚖ 454 KB
Glutathione reductase has been purified to at least 98% homogeneity from calf liver. An essential part in the procedure involves affinity chromatography on 2',5'-ADP-Sepharose 4B to which the enzyme remains bound in the presence of 0.4 M phosphate. This step separates glutathione reductase from the