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Purification of glutathione reductase from erythrocytes by the use of affinity chromatography on 2′,5′-ADP-sepharose 4-B

✍ Scribed by Bengt Mannervik; Kerstin Jacobsson; Vijayakumar Boggaram


Book ID
115906591
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
188 KB
Volume
66
Category
Article
ISSN
0014-5793

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✍ Inger Carlberg; Bengt Mannervik 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 454 KB

Glutathione reductase has been purified to at least 98% homogeneity from calf liver. An essential part in the procedure involves affinity chromatography on 2',5'-ADP-Sepharose 4B to which the enzyme remains bound in the presence of 0.4 M phosphate. This step separates glutathione reductase from the