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Purification of secreted α-amylases by immunoaffinity chromatography with cross-reactive antibody

✍ Scribed by S. Katoh, M. Terashima

Book ID
120767909
Publisher
Springer
Year
1994
Tongue
English
Weight
415 KB
Volume
42
Category
Article
ISSN
1432-0614

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Purification of recombinant proteins by
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✍ Jytte Pedersen; Claus Emborg; Jane Sørensen; Kirsten Biedermann 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 609 KB

Nuclease produced by recombinant Escherichia coli was successfully purified by immunoafftnity chromatography using an antibody preselected for the purpose on the basis of an elution assay. The elution assay was also used to optimize elution conditions. One step recovery of nuclease from crude peripl