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Purification of recombinant proteins by immunoaffinity chromatography with preselected antibodies

✍ Scribed by Jytte Pedersen; Claus Emborg; Jane Sørensen; Kirsten Biedermann

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 609 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00156360

No coin nor oath required. For personal study only.

✦ Synopsis

Nuclease produced by recombinant Escherichia coli was successfully purified by immunoafftnity chromatography using an antibody preselected for the purpose on the basis of an elution assay. The elution assay was also used to optimize elution conditions. One step recovery of nuclease from crude periplasmic extract was 66%.

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