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Purification of recombinant α-amylase by immunoaffinity chromatography with anti-peptide antibody

✍ Scribed by S. Katoh, M. Terashima, M. Kuono

Book ID
120768243
Publisher
Springer
Year
1995
Tongue
English
Weight
624 KB
Volume
43
Category
Article
ISSN
1432-0614

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Purification of recombinant proteins by
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✍ Jytte Pedersen; Claus Emborg; Jane Sørensen; Kirsten Biedermann 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 609 KB

Nuclease produced by recombinant Escherichia coli was successfully purified by immunoafftnity chromatography using an antibody preselected for the purpose on the basis of an elution assay. The elution assay was also used to optimize elution conditions. One step recovery of nuclease from crude peripl