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Purification of recombinant α-amylase with immuno-affinity chromatography using monoclonal antibody

✍ Scribed by Kamihira, Masamichi; Taniguchi, Masayuki; Iijima, Shinji; Kobayashi, Takeshi

Book ID
125478848
Publisher
Elsevier Science
Year
1988
Weight
442 KB
Volume
66
Category
Article
ISSN
0385-6380

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An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody