Single-step method of RNA isolation by a
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Piotr Chomczynski; Nicoletta Sacchi
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Article
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1987
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Elsevier Science
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English
⚖ 414 KB
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of sa