✦ LIBER ✦

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

✍ Scribed by Piotr Chomczynski; Nicoletta Sacchi

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 414 KB
Volume: 162
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90021-2

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✦ Synopsis

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples. 0 1987 Academic

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