A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of sa
โฆ LIBER โฆ
Single-Step Method of Total RNA Isolation by Sodium Dodecyl Sulfate/Phenol Extraction from Cultured Cells
โ Scribed by Jubao Duan; Xin Cai; Limei Zhou; Jiaxi Wang
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 114 KB
- Volume
- 251
- Category
- Article
- ISSN
- 0003-2697
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A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonatesodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium