✦ LIBER ✦

Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

✍ Scribed by Daniel S.H. Liu; Glenn Hiltibran; Thomas Castle; Arlan Richardson

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 309 KB
Volume: 88
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90468-2

No coin nor oath required. For personal study only.

✦ Synopsis

A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonatesodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.