The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Single-step method of RNA isolation by a

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

✍ Piotr Chomczynski; Nicoletta Sacchi 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 414 KB

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of sa