𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of rat liver lysosomal α-glucosidase

✍ Scribed by C. Dissous; J.F. Ansart; A. Cheron; J. Krembel

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
571 KB
Volume
116
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Lysosomal

u-glucosidase (EC 3.2.1.20) was purified from a lysosome-enriched fraction of rat liver using an improved procedure.

A purification factor of 2900-fold was reached, with a yield of 35%. Polyacrylamide gel electrophoresis of the purified enzyme in nondenaturing conditions, or in the presence of SDS, showed only one hand. However, a microheterogeneity among enzyme subunits was detected by high-resolution two-dimensional electrophoresis.

📜 SIMILAR VOLUMES

Lysosomes of rat liver and dab carcinoge
Lysosomes of rat liver and dab carcinogenesis
✍ Tatsuya Takano; Nobuo Kato; Setsuko Kunimoto-Miyata; Sataro Goto; Shoji Ohkuma; 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 539 KB

## Abstract The relationship between DAB carcinogenesis and changes of lysosomes in rat liver was investigated. After 40 days of treatment with DAB, nodules and an abnormal cell population, containing small, large and degenerative cells, appeared. Corresponding to these changes, the S/P ratio of ly

Purification of branched chain α-ketoaci
Purification of branched chain α-ketoacid dehydrogenase complex from rat liver
✍ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s